human circrna array v2 (8x15k)  (Arraystar inc)

Journal: International Journal of Biological Sciences

Article Title: Intergenic CircRNA Circ_0007379 Inhibits Colorectal Cancer Progression by Modulating miR-320a Biogenesis in a KSRP-Dependent Manner

doi: 10.7150/ijbs.85063

Figure Lengend Snippet: Downregulated circ7379 is associated with CRC progression. ( A ) Volcano plot showing the expression profile of 3 CRC tissues and matched normal tissues. Conditions for screening differences: │Fold Change│>1.5, P <0.05. The red points in the plot indicate significantly upregulated circRNAs, and the green points indicate significantly downregulated circRNAs. ( B ) Heatmap showing the top 10 downregulated circRNAs in CRC tissues. High values are shown in red, while low values are shown in green. Each column indicates one sample, and each row indicates one circRNA. ( C ) qRT‒PCR showing the expression of selected circRNAs in 10 pairs of CRC tissues and adjacent normal tissues. ( D ) qRT‒PCR showing the expression of circ7379 in 45 pairs of CRC tissues and adjacent normal tissues. ( E ) qRT‒PCR showing the expression of circ7379 in 25 paracancerous normal tissues, 25 stage I+II CRC tissues, and 30 stage III+IV CRC tissues. ( F ) qRT‒PCR showing the expression of circ7379 in a normal colon cell line (FHC) and a series of CRC cell lines (HT29, RKO, HCT116, SW480, SW620, LoVo, HCT8 and LS123). The data are shown as the mean ± SD. The P values were determined by a two-tailed paired (C and D) or unpaired Student's t test (E and F); * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001. See also .

Article Snippet: A circRNA microarray analysis was carried out for the genome-wide profiling of circRNAs in CRC tissues using an Arraystar Human circRNA Array V2 (8x15K, ArrayStar, Rockville, MD, USA) by a contract service at KangChen Biotech (Shanghai, China).

Techniques: Expressing, Two Tailed Test

Journal: International Journal of Biological Sciences

Article Title: Intergenic CircRNA Circ_0007379 Inhibits Colorectal Cancer Progression by Modulating miR-320a Biogenesis in a KSRP-Dependent Manner

doi: 10.7150/ijbs.85063

Figure Lengend Snippet: Circ7379 is an intergenic circRNA localized in the nucleus and cytoplasm. ( A ) qRT‒PCR showing the amplification of circ7379 from cDNA or gDNA of CRC cell lines using divergent primers and convergent primers. cDNA, complementary DNA; gDNA, genomic DNA. ( B ) Agarose gel electrophoresis of PCR products showing the existence and circulation of circ7379 in CRC cell lines. ( C ) Sanger sequencing showing the back-splice junction of circ7379 . ( D ) RT‒PCR using random primers or oligo dT primers showing the circular characteristics of circ7379 . GAPDH was used as a control for a linear RNA transcript. ( E ) qRT‒PCR showing the expression of circ7379 and GAPDH mRNA after treatment with RNase R in CRC cell lines. ( F ) qRT‒PCR showing the expression of circ7379 and GAPDH mRNA after treatment with actinomycin D at the indicated time points in CRC cell lines. ( G ) RNA fluorescence in situ hybridization (FISH) showing the location of circ7379 in CRC cell lines. Nuclei were stained with DAPI. Scale bar, 10 µm. ( H ) Cytoplasmic and nuclear mRNA fractionation experiments showing the location of circ7379 in CRC cell lines. U6 and GAPDH were used as positive controls in the nucleus and cytoplasm, respectively. The data are shown as the mean ± SD. The P values were determined by a two-tailed unpaired Student's t test (D and E) or two-way ANOVA (F); * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001. See also .

Article Snippet: A circRNA microarray analysis was carried out for the genome-wide profiling of circRNAs in CRC tissues using an Arraystar Human circRNA Array V2 (8x15K, ArrayStar, Rockville, MD, USA) by a contract service at KangChen Biotech (Shanghai, China).

Techniques: Amplification, Agarose Gel Electrophoresis, Sequencing, Control, Expressing, Fluorescence, In Situ Hybridization, Staining, Fractionation, Two Tailed Test